Infecting DCs
Protocols Involving Dendritic Cells: Infecting Dendritic cells with LP In this experiment, we will infect dendritic cells which have been obtained from the bone marrow of mice. See on how to…
Cell Concentration
Measuring Cell Concentrations Question (Concentrations: cells): You want to split cells into a new flask so that the flask with have a 50 ml volume and final cell concentration of 4…
Molt 4 Cells
Unthawing Cells: (1) Obtain 2 50 ml tubes; place 10% C RPMI media in to one tube as working stock (2) before cells are completely thawed, use 2 ml pipette…
MT-2 Cells
Cell Mediums RPMI + 1X P/S + 1X L-glu without serum (RO) take the RPMI and dilute 1:100 with streptamycin and glutamine Thawing Cells Reagents: RPMI media (Roswell Park Memorial Institute)…
Cell Thawing
Companies: BioLife Solutions For thawing cells, conventional practice is to warm the cells quickly in a warm water bath (e.g., 37 C) to just about the point to which the…
Dendritic cells as regulators of T regs
US Patent Pub # 20040109851; Use of immature dendritic cells to selence antigen specific cd8cell function Immature DCs: Another way to generate suppressor T cells in vitro involves the stimulation of…
Dendritic Cell Development
See also Drugs/agents which inhibit DC maturation DC are derived form bone-marrow progenitors (CD34+) and are present at two stages in the body. DC differentiation involves the change from multipotential…
JamesRogers
About the Author: James Rogers is a patent attorney and former patent examiner with the United States Patent and Trademark Office. Previous to his work as a patent examiner, James was…
Capillary electrophoresis
Companies: Biotechne Simple Western (after preparing reagents they are loaded into a Simple Western plate which is placed with a cpaillary cartridhe into a Simple Western instrument. The Simple Western does…