Linkers
Attachment of Protein A to a solid support, Generally Methods of attaching protein ligands sucha s protein A and G to a solid support include ativating a media with a…
Blood Purification
Companies: Haemonetics Apheresis (cytapheresis and Plasmapheresis): Conventional apheresis methods include centrifugation, which can separate any of the components of the blood, and membrane filtration, which can be used for plasmapheresis…
Protein L
See also Continuous Chromatography under Chromatography, Column arrangement. Commercial Protein L Resins: Capto L (GE Healthcare Life Sciences) Data file 29-0100-08 AB) combines a rigid, high flow agarose matrix with…
By Hydrophobic Resins & Activated Carbon
See also Carbon Filters Companies: Merk Group: Merck KGaA (subsidiaries: EMD Millipore Corporation) Separation of Antibodies using Hydrophobic Chromatography Resins Polystyrene or poly(ethyl)styrene or Vinylbenzene: --cross-linked with Copolymers od divinylbenzene…
antibody production techniques
Companies: AI screening with AI: OminiAb complete human antibodies: Infinimmune Production of Antibody Fragments: Production of Light chains dimers in E. coli: Rognoni ("A strategy for synthesis of pathogenic human…
therapeutic applications for antibodies
See also Cancer specific antibodies . See also Wikipedia "List of Therapeutic monoclonal antibodies" Commercially approved antibodies: See the Immunology Link. web database of mAbs with clinical indications Examples include: (Rituximab or…
Cell expression of Antibodies
Introduction -Definitions: hybridoma fusion and cloning supplement (HFCS): is a low serum containing media supplement for the repalcemetn of fetal calf serum (FCS) in cultures of hybridoma cells. HFCS is specifically…
Antibody Structure and Types
Figure from Nilsson (US6,740,734B1) Introduction: It is well documented that the structure of an immunogloublin forms a Y-shaped glycoprotein that is composed of two identical heavy and two identical light…
Protein G
Commercial Matrixs of Protien G Protein-G Sepharose 4 Fast Flow: is manufactured by GE Healthcare (Hober, J. Chromatogr B, 2007, 848, pp. 40-47 and contains SpG immobilized as a ligand. Introduction…
Polymer precipitation
See also Plasma Protein Precipitation by Various Agents Nonionic, water-soluble polymers induce protein precipitaiton by excluding water from the solvation structure of a protein. PEG is the most widely studied…