Depth Filtration
companies: 3M filtration See also depth filters generally Despite low shear centrifuge designs, the centrate effluent may still contain appreciable amounts of smaller sized contaminants (e.g., aggregates and colloids). Depth…
Fatty acids (e.g., caprylic acid)
See also Virus inactivation (caprylic acid can be used as an agent to do this) See also adjustment of pH with various agents under Agents used for fractionation See also AEX for…
Immobilized Metal ion Affinity chromatography (IMAC)
Commercially available IMAC columns: Profinity IMAC resins: The Profinity IMAC bead is a 60 um particle derivatized with iminodiacetic acid (IDA) which functions as the chelating ligand. The chemical structure of…
Depth Filters
See also depth filtration for antibody purification Companies: 3 M Purification Depth filters refers to the use of a porous medium that is capable of retaining particulates throughout its matrix…
In-Line or Tandem
See also continous or SMB chromatography In General: Brown (WO2010/019148) discloses membrane chromatography run on either a standard chromatography system or a custom system like an AKTA exploer (GE Healthcare)…
Buffers
A buffer is a solution that resists changes in pH by the action of its acid-base conjugate components. Buffers act by releasing hydrogen ions when a base is added and…
Inclusion bodies
As to prokaryotic systems for the production of antibodies see Ypatent Health Definitions: Chaotropic agent: refers to a compound that in a sutiable concentraiton is capable of chaing the spatila…
HCIC
See also "purification schemes" under Antibody Purification. See also "mixed mode chromatography" under Chromatography Classification: HCIC is a type of mixed mode chromatographic process in which the protein of interest in the…
Cleveland Clinic — Diagnostic claims
Clevland Clinic Foundation v. True Health Diagnostics (Fed. Cir 2017) applies the two-step Alice framework to several patents of Cleveland Clinic and finds each patent invalid under the framework. US…
Bi-specific antibodies
See also bi-specific antibody production Bispecific antibodies can bind two different antigens. IgG type antibodies have two binding sites with different variable regions. An IgG variable region is made up…