Posts by Super User

See also "Protein A" under "affinity chromatography"  See also Conditions for Affinity chromatography   As to purification of antibody conjugates (e.g., Fc-fusion proteins) see "antibody conjugates"  Affinity separation relies on the specific recognition between…

Commercial Viral Filters: Viresolve® See also Purification of plasma proteins, particularly virus inactivation.  Operating Conditions Addition of Amino Acids: Hongo (US 13/260419) discloses a method of removing viruses from monoclonal antiboides…

High Performance tangential flow filtration (HPTFF): is where the protein separations are carried out on the basis of both size and charge (US 2003/0229212). HPTFF is thus a two-dimensional purification method which…

Tangential Flow Filtration (TFF or crossflow filtration) refers to a filtration process where the feed stream passes parallel to the membrane face as one portion passes through the membrane (permeate)…

The following are commonly used modes of filtration. 1. Direct Flow Filtration (DFF): or "dead-end" filtration is where the feed stream is applied perpendicular to the membrane face, attempting to pass…

Ultrafiltration is a form of membrane filtration in which hydrostatic pressure forces a liquid against a semipermeable membrane. Suspended solids and solutes of high molcular weight are retained, while water and…

Depth filters refers to the use of a porous medium that is capable of retaining particulates throughout its matrix rather than just on its surface. These filters are frequently employed…

See also graft chains and ionic functional groups attached to membranes (under "chromatography" and "graft chains") Types of Materials Used Hollow fiber: In ultrafiltration applications, one very common membrane form for…