Specific Plasma Proteins
Most plasma proteins have their isoelectric point below pH7 so that their solubility will decrease with acidification. Major Human Plasma Proteins (67% of total plasma protein mass) Albumin: serves important functions…
silicon dioxide
Condie, (US 3,998,946) teaches treating blood plasma products with fumed colloidal silica to remove fibrinogen and plasminogen. According to the procedure, blood is centrifuged to separate out the precipitate, the supernantant (plasma)…
ethanol precipitation
Human blood plasma is conveniently fractionated by the cold ethanol precipitation method. For this method the plasma is treated with ethanol at below 5C and by increasing the concentration of ethanol and reducing the pH,…
agents used
See also Antibody precipitation under Antibody purification See also IVIG for use of ethanol as fractionation agent The main fractionation agent used to isolate immunoglobulins from donor plasma is ethanol fractionation based…
Definitions for Plasma Protein Purificaiton
Blood: Blood cells make up about 45% of adult blood. These cells include oxygen carrying erythrocytes (red blood cells), immune cells caled leukocytes (white blood cells) and thrombocytes (platelets). Whole…
fractionation procedures
For more details on the Cohn Alcohol Fractionation Procedure with respect to purifying immunoglobulin (IVIG) see "alcohol precipitation" right hand panel. Common plasma fractionation processes are the Cohn fractionation method (Cohn,…
Plasma Proteins
See also Precipitation of antibodies, Fractionation of plasma and Recovery of concentrates and Types of Plasma Protein fractions Companies: Takeda Collected human plasma may be used as a source material for…
PEG
PEG/Salt Systems PEG-salt two phase system: has been known for some time. Kohler formed 7.5% w/w PEG 1500 and 14% potassium phasphate two phase systems directly in a bioreactor and used…
EOPO
For antibody purification using partitioning see "antibody purification" Many thermoseparating polymers contain ethylene oxide groups. PEG is a thermospearating polymer but its cloud point is too high (above 100C) for use in…
Extraction/partitioning
As to partitioning of antibodies see "purification of antibodies" Extraction is the prcoess of moving one or mroe components of a mixture form one phase to another (compare to precipitation which…