Posts by Super User

General Principles (Complex Interactions): Hydroxyapatite (HA) chromatography of proteins involves the non-specific interaction of the charged amino or carboxylate group of a protein with oppositely charged groups on the hydroxyapatite,…

Cation Mixed Mode (Anion exchangers)/Cation with hydrophobic interaction: Positively charged hydrophobic ligands belong to the group of anion exchanger mixed mode (for example Capto MMC) (for example Capto MMC) (Melinda,…

Hydroxyapatite (HA) See "hydroxyapatite" Hydrophobic charge induction chromatography (HCIC): See "affinity chromatography".  Anion exchange and hydrophilic interaction: (see Anion Mixed Mode) Anion and hydrophobic: (see Anion Mixed Mode) Cation with hydrophobic interaction: See…

See also Ion Exchange Chromatography and particularly types of resins Mixed Mode Chromatography involves the use of solid phase chromatographic supports that employ multiple chemical mechanisms to absorb proteins or other solutes.…

See also filtration membranes using graft chains with functional groups (under "filtration") The use of ligand "tentacles" or "extenders" to improve protein binding capacity and modify resin selectiveity involves placing a…

See also Graft or Side Chains/extenders/linkers (Reactive Monomers)  The carrier or substrate which is covered in another section (i.e., polysaccharide, polypeptide or silica) is modified by a synthetic polymer which…

References for Commercial Resin types (Tables which include the commercial name/vendor/type of resin): (Ramasubramanyan (US 13/829989) See Tables from Fahrner et al. (US 2003/0229212A1) For another table which lists types of…

Controlled pore glass (CPG) is a media used as a backbone matrix for chromatographic resins. CPG is generally produced form a borosilicate base material that is heated to separate the…

See also "controlled pore glass" as an example of silica in the outline A well described matrix for the purificaiton of proteins is silica. It is commercially available in various…

Typical carrier materials include polysaccharides, polypeptides and silica (Hou, US 4,639,513).  Carbonaceous materials Modified carbonaceous materials:  Kyrildis (US2002/0056686) teaches that carbonaceous materials such as  carbon black, graphite and activated carbon, represent an important…