Biochemistry

The glycosyltransferases are a family of some 250-300 different intracellular, mebrane-bound enzymes that participate in the coordinate biosynthesis of the glycostructures of polypeptides, including glycoproteins, proteoglycans and glycolipids. They are…

Serine proteases are a large family of proteoytic enzymes that include digestive enzymes, trypsin and chymotrpsin, components of the complement cascade and of the blood clotting cascade, and enzymes that…

Mechanisms Proteases (also termed "proteolytic enzymes" "peptidases" or "proteinase") attack their substrates using 2 approaches. Exopeptidases remove single residues or dipeptides sequentially from either the N-terminus or C-terminus depending on…

Introduction to CRISPR/Cas9 Companies: Intellia Aldevron (Danaher) Non-profits: Innovative Genomic Institute  Innovative Genomics Insttitute Education Section  Biopku CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR assocaited) systems are prokaryotic adaptive immune systems…

Companies: Beam Therapeutics Caribou Biociences,  CRISPR Therapeutics, Editas Medicine,  Intellia Therapeutics, Poseida, Prime Medicine, Sangamo Therapeutics, Wave LifeSciences, Verve Therapeutics Introduction: CRISPR Cas systems are a main protective mechanism of…

CRISPR (see outline) Restriction Enzymes Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence, called the restriction site. Usually a restriction site is a palindromic…

Alpha-Amylases: constitute a class of enzymes syntheized by a variety of organisms from bacterial to fungi to humans that break down large molecules known as polysaccharides. Polysaccharides, such as starch and…

The glycosylation pattern of immunoglobulins (i.e, the saccharide composition and multitude of attached glycostructures) has a strong influence on the biological properties. Glycosylation of IgG has been shown to be…

See also Methods used to separate antibody glycovariants See also Mass Spectrometry (MALDI TOF MS) Detection and Characterization of product associated variants during the product of polypeptides (e.g., antibodies) is important because profiling…

Definitions Glycan: refers to the carbohydrate portion of a glycoconjugate, such as a glycopeptide, glycoprotein, glycolipid or proteoglycan. Regnier (US8,568,993)  N-glycans Peptides expressed in eukaryotic cells are typically N-glycosylated on asparagine…