Mass spectrometry
See also analytics & Characterization under antibody purification Introduction to Mass Spectrometry Mass spectrometry (MS) has emerged as a core technique for protein identificaiton and characterisation because of its high…
Western blotting
Western blotting: is used to identify a specific protein in a complex mixture of proteins. The technique exploits both the efficiency of SDS-PAGE to separate a mixture of proteins into distinct…
Microarray technology
Over the past decade, more than 30 organisms have had their genomes completely sequenced, including our own genome. But all of this information does not tell us what all the…
Capillary electrophoresis
Capillary electrophoresis with laser induced fluorescence (CE-LIF): is a method for the direct quantitation of gene expression. Detection and Quantification of Proteins Generally: Gavin (US 2015/0093757) disclsoes capillary electrophoresis methods which…
Protein identification
Mass spectrometry has become the method of choice for the Complex mixtures are separated before their MS analysis.
RT-PCR
PCR: Principles of PCR BonesLab (degenerate PCR) Boehringer (RT-PCR) GeneHunter (Differential Display) Competitive PCR Real Time Primer Sets RealTime Probe & Primer Database Ambion (Real Time PCR basics) TRI REAGENT STEP (1) centrifuge samples for 10 min at 10C at 1100 RPMI …
Factors to consider in PCR
Primer-DNA annealing temperature. This is generally dependent on the primer lenght and order and percentage of the nucleotides present composing the primers. Number of cycles (the number of times that the…
PCR applications
Competitive PCR: is a a type of quantitative PCR. Exogenous target is added to create a competition between templates for amplification. The exogenous target is designed to amplify a smaller fragment…
PCR
Diagrams of PCR techniques PCR is a way to amplify DNA sequences even when they are present at only very small quantities. It is a technique invented by Kary Mullis…
Protein-DNA interaction
Technical Approaches toward identifying Proteins with DNA binding activities An important class of protein-protein interactions is that of proteins interacting with specific nucleic acid sequences, such as in the promoter…