Protein Purification

Commercial Kits:  Pierce Chicken IgY Purification Kit   Gallus Immunotech  Promega Eggstract® Ammonium sulfate or sodium sulfate precipitation: has widely been used as an initial purification step in the isolation of immunoglobulin isotypes from serum…

Chloroform --Water dilution Chloroform: Larsen (US 12/299670 and WO2007/079755) discloses an alternative protocol for IgY isolation from egg yolk. First separate egg white and yolk, add 100 mM sodium phosphate buffer…

See also extraction of IgY by precipitation Initial Extraction All IgY purification methods require an initial delipidation step to extract insoluble lipids and lipoproteins. Among the accepted commercial and laboratory delipidation…

Companies working with Avian Antibodies: Amicus Biotech  Differences and Advantages of Avian Antibodies It is known that birds (e.g., laying hens) transfer their immunity to the yolk of their eggs and…

Affinity chromatography (Protein A/G) Affinity - IEX:  Herrmann (WO 2005/100394A2) discloses a process for purifying IL-15/Fc fusion protein by applying the composition to an affinity chromatography column and applying the…

Isotypes as Particular Types of Antibodies Purified and Techniques Used: Antibody Fragments: (for Single Domain Binding Polypeptides and Nanobodies see "domain antibodies" in outline) --Advantages of Using Antibody Fragments:  Small…

For purification of other proteins from milk (which may involve same or similar steps) see Non-Antibody purification For purificaiton of antibodies and other proteins from Inclusion bodies see outline Separation of…

Conditions, such as solution pH, ionic strenght, solvent compoisition, termpature, light and electric field. These polymers are referred to as stimuli response, enivornmentally sensitive, "intelligent" or "smart" polymers. a large…

Co-precipitation of non-IgG contaminants with positvely chartged polymers parallels the selectivity of AEX. These reagents selectively co-precipitate acidic host cell protins, DNA and varous cell culture additivies. (Pete Gagnon, J.…

Anionic polyelectrolytes (polyanion polyelectrolytes)  include copolymers of acrylic acid, methacrylic acid and methyl methacrylate (US 8362217), polyacrylic acid (PAA), polystyrenesulfonic acid (PSS, poly(4-vinylbenzenesulfonate metal soat)), polymethacrylate (PMA), polyacrylamidomethylpropanesulfonate (PAMPS), carboxymethylcellulose (CMC),…