PCR applications
Competitive PCR: is a a type of quantitative PCR. Exogenous target is added to create a competition between templates for amplification. The exogenous target is designed to amplify a smaller fragment…
PCR
Diagrams of PCR techniques PCR is a way to amplify DNA sequences even when they are present at only very small quantities. It is a technique invented by Kary Mullis…
Protein-DNA interaction
Technical Approaches toward identifying Proteins with DNA binding activities An important class of protein-protein interactions is that of proteins interacting with specific nucleic acid sequences, such as in the promoter…
Gene analysis
The analysis of gene expression is a common experiment conducted routinely in the laboratory. Profiling gene expression provides vlauable insight into genes involved in normal cell/tissue homeostasis, organisms development and…
Sample ELISA
IL-12 p40/p70 ELISA 1. Dilute capture anti-IL-12p40/p70 purified (10 ug/ml) in 0.1 M NaHCO3 (pH 8.2) and add 50 ul to appropriate # of weels of Coster EIA medium binding…
Macrophage ELISA
protocol Elisa macrophages obtain Peritoneal macrophages from BALB/c Mice. 1) inject mice with 3 ml thioglycolate broth (encourages macrophages to come to cavity) 2) wait 4 days 3) inject PBS Split cells…
ELISA
Enzyme-linked immunosorbent assay (ELISA), is a popular format of a "wet-lab" type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay (EIA) to detect the presence of a substance in a…
Imunoagglutination
Latex aggluination: is a method for rpaidly measuring a substance to be analyzed, by visually or optically detecting a degreer of latex-particle-allutination caused by a reaction of the substance to be…
Hemagglutination
See also ABO blood group typing Hemagglutination is a general term applied to any agglutination test in which clumping of red blood cells indicates a positive reaction. Human blood can vary…
Agglutination assays
Agglutination Reactions are similar in principle to precipitation reactions in that they depend on the crosslinking of polyvalent antigens. Hemagglutination is used in blood typing. RBCs are mixed on a slide with…