Cell Freezing and Storage
Introduction: The long-term preservation of suspension of cells by freezing and stroage at cryogenic temperatures is a well-established technique. A wide variety of single cell types in addition to multicellular…
Corneal Endothelial Cells
Cell Culutring, Harvesting & Preservation: Kinoshita (US 2023/0380417) discloses a storage method for HCECs or their precursor cells by culturing the cells in a medium that contains a ROCK inhibitor…
Macrophages
Macrophages are specialist cells that have developed an innate capacity to recognize subtle differences in the structure of cell surface epressed identification tages, so called molecular patters. They arrive later to…
Cell Culturing
Common Cell Types: Polypeptides for use in pharmaceutical applications are mainly produced in mammalian cells such as CHO cells, NSO cells, Sp2/0 cells, COS cells, HEK cells, BHK cells, PER.C6® cells…
Infecting DCs
Protocols Involving Dendritic Cells: Infecting Dendritic cells with LP In this experiment, we will infect dendritic cells which have been obtained from the bone marrow of mice. See on how to…
Cell Concentration
Measuring Cell Concentrations Question (Concentrations: cells): You want to split cells into a new flask so that the flask with have a 50 ml volume and final cell concentration of 4…
Molt 4 Cells
Unthawing Cells: (1) Obtain 2 50 ml tubes; place 10% C RPMI media in to one tube as working stock (2) before cells are completely thawed, use 2 ml pipette…
MT-2 Cells
Cell Mediums RPMI + 1X P/S + 1X L-glu without serum (RO) take the RPMI and dilute 1:100 with streptamycin and glutamine Thawing Cells Reagents: RPMI media (Roswell Park Memorial Institute)…
Cell Thawing
Companies: BioLife Solutions For thawing cells, conventional practice is to warm the cells quickly in a warm water bath (e.g., 37 C) to just about the point to which the…