different forms
Calcium-derivatized apatities The inoic state of HA columns can be varied by changing the ionic nature of the buffers with which they are equilibrated. Thus, equilibration with PO4 buffer should…
hydroxapatite
General Principles (Complex Interactions): Hydroxyapatite (HA) chromatography of proteins involves the non-specific interaction of the charged amino or carboxylate group of a protein with oppositely charged groups on the hydroxyapatite,…
cation mixed mode (anion exchanger)
Cation Mixed Mode (Anion exchangers)/Cation with hydrophobic interaction: Positively charged hydrophobic ligands belong to the group of anion exchanger mixed mode (for example Capto MMC) (for example Capto MMC) (Melinda,…
types of ligands and supports
Hydroxyapatite (HA) See "hydroxyapatite" Hydrophobic charge induction chromatography (HCIC): See "affinity chromatography". Anion exchange and hydrophilic interaction: (see Anion Mixed Mode) Anion and hydrophobic: (see Anion Mixed Mode) Cation with hydrophobic interaction: See…
mixed mode
See also Ion Exchange Chromatography and particularly types of resins Mixed Mode Chromatography involves the use of solid phase chromatographic supports that employ multiple chemical mechanisms to absorb proteins or other solutes.…
graft chains
See also filtration membranes using graft chains with functional groups (under "filtration") The use of ligand "tentacles" or "extenders" to improve protein binding capacity and modify resin selectiveity involves placing a…
types of ligands
See also Graft or Side Chains/extenders/linkers (Reactive Monomers) The carrier or substrate which is covered in another section (i.e., polysaccharide, polypeptide or silica) is modified by a synthetic polymer which…
trade names
References for Commercial Resin types (Tables which include the commercial name/vendor/type of resin): (Ramasubramanyan (US 13/829989) See Tables from Fahrner et al. (US 2003/0229212A1) For another table which lists types of…
controlled pore
Controlled pore glass (CPG) is a media used as a backbone matrix for chromatographic resins. CPG is generally produced form a borosilicate base material that is heated to separate the…
silica
See also "controlled pore glass" as an example of silica in the outline A well described matrix for the purificaiton of proteins is silica. It is commercially available in various…