inteferon purification
Natural interferon production has traditionally involved ammonium chloride treatment of buffy coats to lyse the red blood cells and to isolate the leukocytes, followed by viral stimulation of leukocytes with…
enzyme purification
Glutamic-oxalacetic transaminase: Cammarata "Fractionation and properties of glutamic-oxalacetic transaminase" (1951) teaches that low temperature alcohol fractionation, so successful in plasma protein fractionation, offers advantages over ammonium sulfate in the purification of…
Non-antibody purification
See American Water Works Association See also Virus Separation Definitions Detergents: Detergents are amphipathic molecules, meaning they contain both a nonpolar "tail" having aliphatic or aromatic character and a polar "head". Ionic…
operating conditions
Bind and Elute Mode of Operation A. Equilibrium buffer: Sun (US8058407) discloses a method of purifying at least one acidic protein by applying an equilibration buffer of CaCl2 to HA,…
different forms
Calcium-derivatized apatities The inoic state of HA columns can be varied by changing the ionic nature of the buffers with which they are equilibrated. Thus, equilibration with PO4 buffer should…
hydroxapatite
General Principles (Complex Interactions): Hydroxyapatite (HA) chromatography of proteins involves the non-specific interaction of the charged amino or carboxylate group of a protein with oppositely charged groups on the hydroxyapatite,…
cation mixed mode (anion exchanger)
Cation Mixed Mode (Anion exchangers)/Cation with hydrophobic interaction: Positively charged hydrophobic ligands belong to the group of anion exchanger mixed mode (for example Capto MMC) (for example Capto MMC) (Melinda,…
types of ligands and supports
Hydroxyapatite (HA) See "hydroxyapatite" Hydrophobic charge induction chromatography (HCIC): See "affinity chromatography". Anion exchange and hydrophilic interaction: (see Anion Mixed Mode) Anion and hydrophobic: (see Anion Mixed Mode) Cation with hydrophobic interaction: See…
mixed mode
See also Ion Exchange Chromatography and particularly types of resins Mixed Mode Chromatography involves the use of solid phase chromatographic supports that employ multiple chemical mechanisms to absorb proteins or other solutes.…
graft chains
See also filtration membranes using graft chains with functional groups (under "filtration") The use of ligand "tentacles" or "extenders" to improve protein binding capacity and modify resin selectiveity involves placing a…