Batch Chromatography
Batch Chromatography Traditionally, capture chromatography is run in single column, discontinous mode which is characterized in that the column is consecutively (i) equilibrated, (ii) loaded with feed containing the target…
conditions and modes
See also antibody purification Definitions Ion-exchange chromatography: retention is based on the attraction between solute ions and charged sites bound to the stationary phase. High-perfromance liquid chromatography (HPLC): is a…
expanded bed chromatography
Fluidized bed cchromatography involves the use of adsorbetn particles dispersed in a liquid medium. (Gagnon, J. Chromatogr. A 1221 (2012) 57-70). Expanded bed chromatogrpahy (EBA): EBA is a type of stabilized…
monoliths
Monolithic stationary pahses consist of a porous polymer block. To create the polymer, monomer and cross linker molecules as well as molecules beraing the interactive groups (e.g., an ionomer in…
columns used
Continuous (simulated moving bed) chromatography: See outline As to monoliths see outline Companies: Hosptial Supply Corporation In general, chromatographic methods chromatography columns are employed which are essentially comprising a column…
Definitions
Amphiphilic: contain both hydrophobic groups ("tails") and hydrophilic groups ("heads"). Surfactants (detergents) are typically organic compounds that are amphiphilic which render surfactants soluble in both organic solvents and water. Aqueous solution: refers to a…
Chromatography
See also Helpful Books in Resources: The term chromatography refers to a process by which a solute of interest (e.g., protein of interest) in a mixture is separated from other solutes by…
glycated variants
See also Lectin Affinity Chromatography under Affinity Chromatography See also antibody glycosylation in "biochemistry" under "glycosylation" See also Characterization and Detection of Glycosylation The potential for significant changes in the biological activities…
charge variants
MAbs undergo several posttranslational modifications such as disulfide bond formation, oxidation, deamidation, truncation and cyclization of N terminal glutamine residues to pyroglutamate. The heterogeneity observed in mAbs is often moniteored…
aggregates
Protein aggregation is a common phenomenon that can be encountered during various stages of a commercial antibody manufacturing process. For example, aggregates can form during the fermentation, purification, final formulation…