Hands on Education

Bioprocessing: NC State University

Basic Lab Techniques:

Biologue

Antibodies/Immunology

Antibodies: A Laboratory Manual, 2d ed. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1988)

Current Protocols in Immunology

Kabat et al. (1971) Ann. NY Acad. Sci. 190: 382-391 and Kabat, E. et al. (1991) “Sequences of Proteins of Immunological Interest” fifth Edition, US Department of Health and Human Services, NIH Publication No. 91-3242

Handbook of experimental immunology (D.M. Weir, ed) Alden Press, Oxford

Harlow and Lane, Using Antibodies, Cold Spring Harbor Laboratory Press (1999); Harlow and Lane, Antibodies, A Laboratory Manual, Cold Spring Harbor Laboratory Press (1988).

Chemistry/Protein Conjugation

Bioconjugate Techniques, by Greg T. Hermanson, Academic Press, 2008

Chemistry of Protein Conjugation and Cross-linking, by Shan S Wong, CRC Press, 1991

Nanotechnology:

Gupta “Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications,” Biomaterials (2005) 26: 3995-4021.

Protein Purification/Separation: 

aGenerally

Protein Purification Principles, high-resoultion methods, and applications,1998, 2nd ed., Janson, J-C, Ryden, L, Wiley & Sons Inc., New York

Protein Purification, Ed. Janson JCD, Ryden L, VCH-Verlag, Weinheim, Germany (189)

Protein Purification Methods, A Practical Approach, Ed. Harris E L V, Angal S, IRL Press Oxford England (1989)

Protein Purification: Principles and Practice, Scopes, Springer Verlag, New York, (1982)

Process Scale Bioseparations for the Biopharmaceutical Industry, Ed. Shukla AA, Etzel M R, Gadam S, CRC Press Taylor & Fracis Group (2007),

Protein Purification Handbook, GE Healthcare 2007 (18-1132-29)

Purification Tools for monoclonal Antibodies Peter Gagnon, 1996 ISBN-9653515-9-9.

Chromatography:

Chromatography, 5th edition, Part A: Fundamentals and Techniques, Heftmann, E. (ed.), ENew York (1992); 

Advanced Chromatographic and Electromigration methods in Biosciences, Deyl, Z. (ed.), (1998); Chromatography Today, Poole, C.F. and Poole, S.K., E(1991); 

Sambrook, J., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor (1989); Current Protocls in Molecular Biology, Ausubel, F.M..

Current Protocls in Molecular Biology, Ausubel, F.M., et al., (eds), John Wiley & Sons, Inc., New York

–Reversed-phase high performance liquid chromatography (RP-HPLC)

McDonald and Bidlingmeyer, “Strategies for Successful Preparative Liquid Chromatogrpahy” Preparative Liquid Chromatography, Brian A. Bidingmeyer (New York: Elsevier Science Publishing, 1987), vol. 38, pp. 104

Lee et al. Preparative HPLC. 8th Biotechnology Symposium, Pt. 1, 593-610 (1988)

Filtration

Michaels “Fifteen years of ultrafiltration: Problems and Future promises of an adolescent technology” in Ultrafiltration Membranes and Applications, Polymer Science and Technology, 13 (Plenum Press, N.Y., 1979, Anthony R. Cooper, ed., )

Porter, ed. “Handbook of Industrial Membrane Technology” (Noyes Publications, Park Ridge, N.J., 1990), pp. 164-173

Microfiltration and Ultrafiltration: Principles and Applications, L. Zeman and A. Zydney (Marcel Dekker, Inc., NY, NY 1996)

Ultrafiltration Handbook, Munir Cheryan (Technomic Publishing, 1986; ISBN No. 87762-456-9).

d. Blood/Plasma Purification

Curling, J.M. (Ed.), Methods of Plasma Protein Fractionation, Academic Press Inc., 1980

Lundblad J.L. and Schroeder D.D. “Methds for the Production of IVIG Preparations and Analysis of IVIG Preparations” in Clinical Applications of intravenous immunogolbuine therapy, P.L. Yap, ed., Churchill Livingstone Inc., New York, 1992.

Centrifugation Techniques

Gradient Formation, volume 7 of Centrifugation techniques, Nycomed Pharma AS, 1996

Recombinant DNA technology

J. D. Watson et al., 1992, Recombinant DNA: A Short Course, 2nd ed., W.H. Freeman and Company

Protocols

Current Protocols in Cell Biology

Flow Cytometry and Sorting, 2nd ed. (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds), Academic Press, London 1985

Flow Cytometry: First Principles, 2nd Edition by Alice Longobardi Givan (July 2001)