specific disorders
For therapeutic applications of IL-6 antibodies see IL-6 under "immunology" For cancer specific antibodies see the "cancer" section Cancer and Lymphomas: The first antibody for the treatment of cancer was a chimerica…
bi-specific antibody production & isolation
As to Bispecific antibodies generally see Bispecific antibodies under structure and types As to Be-specific antibody purificaiton see bi-specific antibodies under particular types of antibodies purified see also Engineering of…
monoclonal antibody production
See also Immunization and ways to increase immunogenicity See also B cell activation . See also cell culture in Biotechnology Introduction Since the development of the hybridoma technology by Kohler and Milstein 35…
prokaryotic systems
For the purification of antibodies from inclusion bodies, see Protein Purification and Antibody Purification. Antibody fragments were usually manufactured in mammalian cells, but alternative production systems such as E. coli, Pichia yeasts…
eukaryotic systems
Mammalian Cells: About 60-70% of all recombinant protein pharmaceuticals and 95% of approved antibodies are still produced in mammalian cell lines despite relatively high produciton costs and diffiulty in handling.…
Recombinant gene expression
The development of most manufacturing processes for recombinant proteins such as antibodies in mammalian cells usually follows a a sehme of recombinant gene with the necessary transcriptional regulatory elements being…
Fc receptor modification
Increasing Serum Half-life See engineering antibodies for pH dependent affinity Introduction The Fc region of an antibody interacts with a number of Fc receptors and ligands, imparting an array of important…
pH dependent antibodies
See also Fc Receptors See also FcRn recpetor See also Fc modifications Increasing the half lives of therapeutics can decrease the frequency of patient inections and allow the administration of low concentrations of…
modified for binding affinity
Introduction The targets of monoclonal antibodies can be classified into two groups: membrane-bound antigens and soluble antigens. Targets of food and drug adminsitration (FDA) approved mAbs for membrane boudn antigends…
humanized antibodies
Useful Links: IMGT (International ImMunoGeneTics Information system; useful information on germline gene repertoire) Because mice are convenient for immunization and recognize most human anitgens as foreign, mAbs against human targets…