Embryonic Development
Introduction: Definitions: During embryoic development, cells specialize in carrying out particular functios. In all animals except sponges, the process is irreversible: once a cell deifferentiates to serve a function, it…
Cell Development
Human Cell Atlas (maps every cell of the human body) See also types of cells See also fertilization and embryonic development Introduction: Cell development starts as a fertilized egg (Zygote)…
Cell Freezing and Storage
Introduction: The long-term preservation of suspension of cells by freezing and stroage at cryogenic temperatures is a well-established technique. A wide variety of single cell types in addition to multicellular…
Cell Culturing
Common Cell Types: Polypeptides for use in pharmaceutical applications are mainly produced in mammalian cells such as CHO cells, NSO cells, Sp2/0 cells, COS cells, HEK cells, BHK cells, PER.C6® cells…
Infecting DCs
Protocols Involving Dendritic Cells: Infecting Dendritic cells with LP In this experiment, we will infect dendritic cells which have been obtained from the bone marrow of mice. See on how to…
Cell Concentration
Measuring Cell Concentrations Question (Concentrations: cells): You want to split cells into a new flask so that the flask with have a 50 ml volume and final cell concentration of 4…
Molt 4 Cells
Unthawing Cells: (1) Obtain 2 50 ml tubes; place 10% C RPMI media in to one tube as working stock (2) before cells are completely thawed, use 2 ml pipette…
MT-2 Cells
Cell Mediums RPMI + 1X P/S + 1X L-glu without serum (RO) take the RPMI and dilute 1:100 with streptamycin and glutamine Thawing Cells Reagents: RPMI media (Roswell Park Memorial Institute)…
Surface markers for specific cell types
Macrophages/dendrites/neutrophils: CD14, CD1a and CD64