B cell maturation
Thymus-dependent (TD) activation: B cell activation can occur one of two ways. The first way depends upon TH cells and is called Thymus-dependent (TD). The other way does not and is referred to…
B cell development
See also antibody diversity B cells develop in the bone marrow. B-cell development begins as lymphoid stem cells differentiate into progenitor B cells (pro-B cells) which express a transmembrane tyrosine…
B cell isolation
One of the critical factors underlysing the difficulty in generating human Mabs is the low frequency of antigen specific B cells in the peripheral blood. Typically, antigen specific B cells…
B cell activation
See also antibody production methods B cell activation can occur one of two ways. The first way depends upon TH cells and is called Thymus-dependent (TD). The other way does not and is…
T cell development
The maturation of most T cells and the development of their specific receptors are directed by the thymus and its hormones. This contrasts with B cells which develop in the…
T cell activation assays
Proliferation Lymphoproliferative assay (LPA): largely measures the in vitro proliferative capacity of CD4T cells. Typically PBMC are placed in culture with recombinant protein antigens for 5-7 days and then pulsed with…
T cell repertoire
The TCR[beta] chain consists of variable (V), joining (J), diversity (D) and constant (C) regions encoded by gene segments spanning 685 kilobases of human chromosome seven. Diversity and antigen specificity…
T cell functions
TH cells orchestrate an acquired immune response by promoting intracellular killing by macrophages, antibody production by B lymphocytes, and clonal expansion of cytotoxic T lymphocytes. The TCRs of TH cells…
Naive T cells
Naive T cells: CD4 and CD8 T cells leave the thymus and enter circulation as resting cells in the G0 stage of the cell cycle. These naive cells express high levels…
T cell receptor
The TCR[beta] chain consists of variable (V), joining (J), diversity (D) and constant (C) regions encoded by gene segments spanning 685 kilobases of human chromosome seven. Diversity and antigen specificity…