Protein Purification

See also Mass Spectrometry in Diagnostic Techniques Companies: Refeyn (mass photometry) Definitions Amide: includes a derivative form of carboxylic acid in which the hydroxyl group has been replaced by an amine or ammonia.…

See also purification of bi-specific antibodies under particular antibodies purified In general, IgG type bispecific antibodies are composed of two types of H chains (an H chain for antigen A…

See also simulated moving bed chromatography (this is a form of continuous chromatography) under moving bed.  Definitions: Binding capacity and mass transfer: The binding capacity strongly depends on the affinity…

Attachment of Protein A to a solid support, Generally Methods of attaching protein ligands sucha s protein A and G to a solid support include ativating a media with a…

See also Carbon Filters Companies:  Merk Group:  Merck KGaA   (subsidiaries: EMD Millipore Corporation) Separation of Antibodies using Hydrophobic Chromatography Resins   Polystyrene or poly(ethyl)styrene or Vinylbenzene: --cross-linked with Copolymers od divinylbenzene…

Commercial Matrixs of Protien G Protein-G Sepharose 4 Fast Flow: is manufactured by GE Healthcare (Hober, J. Chromatogr B, 2007, 848, pp. 40-47 and contains SpG immobilized as a ligand.  Introduction…

See also Plasma Protein Precipitation by Various Agents  Nonionic, water-soluble polymers induce protein precipitaiton by excluding water from the solvation structure of a protein. PEG is the most widely studied…

See also Virus inactivation (caprylic acid can be used as an agent to do this) See also adjustment of pH with various agents under Agents used for fractionation See also AEX for…

Commercially available IMAC columns: Profinity IMAC resins: The Profinity IMAC bead is a 60 um particle derivatized with iminodiacetic acid (IDA) which functions as the chelating ligand. The chemical structure of…

See also continous or SMB chromatography  In General:  Brown (WO2010/019148) discloses membrane chromatography run on either a standard chromatography system or a custom system like an AKTA exploer (GE Healthcare)…