Protein Purification

For the Purification of Antibodies using Immunoaffinity of antibodies Particular types of Antibodies Used VHHs:  CaptureSelect technology is proving to be an essential purification platform for many bioterapeutic molecules including viral…

Hydrophobic interaction chromatography (HIC) is frequently used for the purificaiton and analytical characterization of proteins. This chromatographic mode relies on interactions between hydrophobic patches on the protein's surface with immobilized…

(compare hydrophobic charge induction chromatography under "mixed mode chromatography"); See also antibody purificaiton by HIC HIC is useful for the purification and separation of molecules such as proteins based on…

  As to Antibody purification by affinity chromatography see antibody purification .   As to affinity purication of antibodies by FcyRs see antibody purification by affinity chromatography As to antibody purification…

The large-scale, economic purification of proteins is an increasinly important problem for the biopharmaceutical industry. Manufactuers of protein based pharmaceutical products must comply with strict regulatory standards, including extremely strinent…

Introduction: In this mode of chromatography, the product of interest is loaded beyond the dynamic binding capacity of the chromatography material for the product, thus referred to as overload.  In…

Weak partitioning mode of operation enhances the F/T mode by identifying solution conditions wehre there is weak binding of MAb to the resin (2 to 20 g/L). Under these conditions…

Distinguish Overload chromatography (see outline).  See also purification of antibodies using CEX displacement mode chromatography and overload chromatography  See also purification of antibodies using AEX displacement mode chromatography and overload chromatography Displacement chromatogrphy employs…

Batch Chromatography Traditionally, capture chromatography is run in single column, discontinous mode which is characterized in that the column is consecutively (i) equilibrated, (ii) loaded with feed containing the target…

See also antibody purification Definitions Ion-exchange chromatography: retention is based on the attraction between solute ions and charged sites bound to the stationary phase.  High-perfromance liquid chromatography (HPLC): is a…