expanded bed chromatography
Fluidized bed cchromatography involves the use of adsorbetn particles dispersed in a liquid medium. (Gagnon, J. Chromatogr. A 1221 (2012) 57-70). Expanded bed chromatogrpahy (EBA): EBA is a type of stabilized…
monoliths
Monolithic stationary pahses consist of a porous polymer block. To create the polymer, monomer and cross linker molecules as well as molecules beraing the interactive groups (e.g., an ionomer in…
columns used
Continuous (simulated moving bed) chromatography: See outline As to monoliths see outline Companies: Hosptial Supply Corporation In general, chromatographic methods chromatography columns are employed which are essentially comprising a column…
Definitions
Amphiphilic: contain both hydrophobic groups ("tails") and hydrophilic groups ("heads"). Surfactants (detergents) are typically organic compounds that are amphiphilic which render surfactants soluble in both organic solvents and water. Aqueous solution: refers to a…
Chromatography
See also Helpful Books in Resources: The term chromatography refers to a process by which a solute of interest (e.g., protein of interest) in a mixture is separated from other solutes by…
charge variants
MAbs undergo several posttranslational modifications such as disulfide bond formation, oxidation, deamidation, truncation and cyclization of N terminal glutamine residues to pyroglutamate. The heterogeneity observed in mAbs is often moniteored…
aggregates
Protein aggregation is a common phenomenon that can be encountered during various stages of a commercial antibody manufacturing process. For example, aggregates can form during the fermentation, purification, final formulation…
Particular contaminants
As to charge variants see outline Therapeutic proteins can undergo a variety of degradation processess, including aggregation, deamidation, isomerization, oxidation, disulfide bond scrambling and truncation. These events can occur during fermentation,…
domain antibodies
Introduction Small genetically engineered immuoglobical constructs are being developed industry wide for a growing range of in vivo applications. Examples include Fab, F(ab')2, single-chain (sc) Fv, bis-scFV, diabodies, minibodies and…
IgA
See also purification of IgA from plasma Immunoglobulin A is the dominant immunoglobulin in human secretions including breast milk. Due to the low levels of IgA normally present in cows' milk,…