Membrane absorbers
Membrane adsorbers are thin, synthetic, microporous or macroporous membranes that are derivatized with functional groups akin to those on the equivalent resins. The membranes are stacked 10-15 layers deep in…
By filtration
See also Membrane Adsorbers See also filtration in conjunction with Protein A chromatography for the purification of antibodies See also "Anion exchange" for membrane absorbers with anion exchange ligands. Companies: disposable depth filter system: Pall, Cuno,…
Immunoaffinity of antibodies
See also Immunoaffinity generally under Affinity Chromatography Particular Types of Antibodies Used VHH antibodies: VHH which are naturally devoid of light chains can be used to generate immunosorbent materials for…
by HIC
HIC is a well studied major polishing step in the purificaiton process of IgG based products and known for its capability to remove aggregated from of antibody. (Chen, J. Chromatography…
by HCIC
See also HIC and HCIC under "affinity chromatography" Hydrophobic charge induction chromatography (HCIC) is a type of mixed mode chromatography which as advatnages of mild elution conditionk high capacity and salt-toelrance.…
Fc receptors as affinity ligands
As to Fc receptors generally see Fc Receptors in Signal Transduction Fc receptor is a general term that refers to any one of several proteins that bind to the Fc region…
virus inactivation
In General Inactivation of contaminating virus and removal of this virus is an important concern in the medical industry for the production of recombinant protein. (Zou, WO/2008/036899) Low Ph Hold: A…
secondary steps
See also ion exchange and Particular types of antibodies Affinity A followed by AEX Chromatography A typical platform for purificaiton of mAbs derived from recombinant cell culture employes three chromatographic steps,…
conditions
Protein A chromatography exploits the fact that murine IgG binds to Protein A Sepharose at pH>8 but does not bind at pH<3.0. Typically, the pH of the MAb-containing solution is…
protein A/G
Staphylococcus aureus Protein-A (SpA): Protein A affinity chromatography has become well established as the preferred capture step for purification of human monoclonal IgG for in vivo applicaitons, so much so that it has…