Protein Purification

A buffer is a solution that resists changes in pH by the action of its acid-base conjugate components. Buffers act by releasing hydrogen ions when a base is added and…

As to prokaryotic systems for the production of antibodies see Ypatent Health Definitions: Chaotropic agent: refers to a compound that in a sutiable concentraiton is capable of chaing the spatila…

See also "purification schemes" under Antibody Purification.   See also "mixed mode chromatography" under Chromatography Classification:  HCIC is a type of mixed mode chromatographic process in which the protein of interest in the…

See also bi-specific antibody production  Bispecific antibodies can bind two different antigens. IgG type antibodies have two binding sites with different variable regions. An IgG variable region is made up…

See also "Protein A" under "affinity chromatography"  See also Conditions for Affinity chromatography   As to purification of antibody conjugates (e.g., Fc-fusion proteins) see "antibody conjugates"  Affinity separation relies on the specific recognition between…

Commercial Viral Filters: Viresolve® See also Purification of plasma proteins, particularly virus inactivation.  Operating Conditions Addition of Amino Acids: Hongo (US 13/260419) discloses a method of removing viruses from monoclonal antiboides…

High Performance tangential flow filtration (HPTFF): is where the protein separations are carried out on the basis of both size and charge (US 2003/0229212). HPTFF is thus a two-dimensional purification method which…

Tangential Flow Filtration (TFF or crossflow filtration) refers to a filtration process where the feed stream passes parallel to the membrane face as one portion passes through the membrane (permeate)…

The following are commonly used modes of filtration. 1. Direct Flow Filtration (DFF): or "dead-end" filtration is where the feed stream is applied perpendicular to the membrane face, attempting to pass…

Ultrafiltration is a form of membrane filtration in which hydrostatic pressure forces a liquid against a semipermeable membrane. Suspended solids and solutes of high molcular weight are retained, while water and…