silica
See also "controlled pore glass" as an example of silica in the outline A well described matrix for the purificaiton of proteins is silica. It is commercially available in various…
specific types
Typical carrier materials include polysaccharides, polypeptides and silica (Hou, US 4,639,513). Carbonaceous materials Modified carbonaceous materials: Kyrildis (US2002/0056686) teaches that carbonaceous materials such as carbon black, graphite and activated carbon, represent an important…
types of resins
Type of supports Used There are many different ion exchanger separation supports for biopolymers on teh market. The most common are of beaded type. The particles can be packed in…
cation exchange
Introduction: Cation exchange chromatography refers to a solid phase which is negatively charged, and which thus has free cations for exchange with cations in an aqueous solution passed over or…
anion exchange
See also antibody purification using Anion exchange chromatography under antibody purification and then ion chromatography. Anion exchange is a common process in the recovery of monoclonal antibody products and has been shown to…
ion exchange
Agilent Buffering Advisor Software General Principles: Ion exchange uses an insoluble matrix that carries ionic charges that retard the movement of molecules of opposite charge. There are 2 types, one where…
RPLC
General Principles: RPLC separates on the basis of hydrophobicity. As with other HPLC techniques there is a polymeric stationary phase, of for example polystyrene/divinylbenzene. The mobile phase is usually a combination…
Regeneration
The price of Protein A affinity chromatography resins is many times the cost of non-affinity supports. Accordingly Protein A resins are typically recyled by treatment with strong chaotropic solutions (urea…
Methods of coupling
See also multimers of SpA In General and Definitions Cross-Link: is a bond that links one polymer chain to another. They can be covalent or ionic bonds. Many different types of coupling…
Ligands, Linkers and Immobilization, Generally
See Linkers for Affinity Chromatography Types of Linkers: Flexible Linkers: Flexible linkers are usually applied when the joined domains require a certain deree of movement of interaction. They are generally…