virology

Acute Infection:  During this period the mucosa are the dominant site of infection. The gastointestinal tract and other mucosal tissues contain at steady state at least half of the body's…

Despite multiple, repeated exposures to HIV, some individuals never seroconvert or show any sign of infection. A variety of mechniasms are suggested to be associated with this condition, including HIV…

HIV is a retrovirus: it ahs two identical, single-stranded, plus-sense RNA gehnomes that must be converted to double-stranded DNA by reverse transcription before being expressed and replicated. HIV Models:  Murine…

MT-2 Dye Assay:  (Cell Proliferation Kit) 10 ul to each well with MTT Labeling Reagent Incubate at 37.5 C for 4 hours 100 ul Replica Platting: 100 ul of sample…

HIV anti-retroviral drugs: Truvada (emtricitabine/tenofovir disoproxil fumarate) has recently been approved by the FDA for prevention of HIV. It is a fixed dose of two anti-retroviral drugs and marketed by Gilead…

1) Obtain ELISA plate (this is a special plate which absorbs protein). To 1/2 the wells you will add the buffer and protein (ex. GP160) and the other half of…

Tests for Virus infection: --PCR Viral genome can be applied. Even allows quantification of virus. But due to high sensitivity of PCR can rarely have false negatives particularly where concentration of…

HIV Entry, Integration and Expression HIV infects CD4+ T cells and cells of macrophage lineage. It causes lytic and subsequently latent infection of CD4 T cells (as well as syncytia…

Cross-references to this Web Site: HIV Treatment Strategies HIV abnormalities and symptoms Web Sites: NIAID Division of AIDS (DAIDS) Home Page UN-AIDS HIV molecular immunology database Companies which are involved with HIV: Celera Diagnostics  Calimmune  Sangamo BioSciences  In…

Retroviruses are enveloped, positive  . The virion enclosing the capsid contains 2 copies of the RNA genome. Replication proceeds through a DNA intermediate (provirus). There are 3 following subfamilies of human…