Calthrin-coated vesicles: The major protein component is clathrin. A second major coat protein of clathrin coated vesicles is adaptin which is required to bind the clathrin coat to the membrane and to trap various transmembrane proteins including transmembrane receptors (called “cargo receptors)” that capture soluble cargo molecules. Clathrin coated vesicles budding from different membranes use different adaptins which are specific for different cargo receptors. 

As a clathrin coated bud grows, soluble cytoplasmic proteins, like dynamin (which is a GTPase), assemble a ring around the neck of each bud. Dynamin recuits other proteins and assist in bending the membrane. 

Once the vesicle is released from the membrane, the clathrin coat is lost. A chaperone protein of the hsp70 family functions as an uncoating ATPase, using the energy of ATP hydrolysis to peel off the coat.