See also extraction of IgY by precipitation

Initial Extraction

All IgY purification methods require an initial delipidation step to extract insoluble lipids and lipoproteins. Among the accepted commercial and laboratory delipidation techniques, the most commonly deployed involve either the use of water dilution, chloroform-PEG, polyanionic polysaccharides such as xanthan, dextran sulpate or other natural gums such as pectin. Tan (J Immunological Methods, “A novel, cost-effective and efficient chicken egg IgY purificaiton procedure” 2012).

UF/DV

Hernanez-Campos (J Agric Food Chem, 2010, 58 187-193) discloses purification of IgY from hen egg yolk water soluble protein fraction by UF/DF. The best results were obtained in the absence of salt at pH values 5.7 and 6.7 using poliethersulfone (PES) and modified PES.