(1) get w black agar plates (Jimmy makes it); (use 2 plates just in case 1 does not grow)  label LP on back of plate with Date and strain #. The vials are in last shelve of freezer (use Lp past through thioglycolated AJ Mqs. Swabs are in needles and swab drawer. 

(2) Dip sterile swab into vial (or pour entire contents of vial into plate if culturing only 24 hours. then use swab to spread it around on plate and use same swab to streak other plate) 

(3) streak (long) onto agar

(4) culture for 48 hours upside down (place in lower incubator).

(5) clean up area and use UV light for 10-15 min.