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Antibody Purification
Antibody purificaiton schemes
Affinity chromatography of antibodies
specific affinity ligands
protein A/C
conditions
secondary steps
virus inactivation
Fc receptors as affinity ligands
by HIC
Immunoaffinity of antibodies
Peptides and Mimotopes
By Hydrophobic Resins & Activated Carbon
By filtration
Membrane absorbers
carbon filters
Depth Filtration
By ion exchange
AEX
CEX
by mixed mode chromatography
by hydroxyapatite
by HCIC
by partitioning
by precipitation
affinity precipitation
polyelectrolyte
Anionic
Cationic
Polymer precipitation
smart polymers
particular types of antibodies
antibody fusion proteins
Avion antibodies
IgY isolation
Precipitation agents
ammonium sulfate
IgA
domain antibodies
bi-specific antibodies
Particular contaminants purification
aggregates
charge variants
glycated variants
Extra Light Chains
Oxidized and Reduced Variants
Particular Sources for purification
Inclusion bodies
bioterrorism
Common agents of bioterrorism
Analytics & Characterization
next generation sequencing
Common Techniques
Light scattering
Mass spectrometry
Capillary electrophoresis
Plasma Proteins
fractionation procedures
Definitions
agents used
silicon dioxide
Fatty acids
Specific Plasma Proteins
albumins
IVIG
ethanol fractionation
ethanol precipitation Steps
chromatography
anion exchange of plasma proteins
cation exchange of plasma proteins
commercial preparations
diagrams
virus inactivation
aggregate/enzyme removal
IgM
IgA
Factor H
Protease Inhibitors
Inter-alpha-Inhibitor Proteins
A1PI
Chromatography
Definitions
Buffers
Column Arrangement or Use
Monoliths
expanded bed chromatography
In-line or Tandem Chromatography
Blood Purification
Batch Chromatography
continuous chromatography
Moving Bed
conditions and modes
displacement chromatography
weak partitioning mode
Overload Chromatography
purification schemes
Affinity Chromatography
IMAC
Lectin Affinity Chromatography
Immunoaffinity
Protein A
Structure
Analogues of Protein A
Temperature senstive mutants
Multimers of SpA domains
Parameters
elution
Resins
methods of coupling ligands to resin
Linkers
Regeneration
Protein L
Protein G
HIC
Conditions for HIC
RPLC
Small Molecules
Ion-Exchange Chromatography
Anion Exchange Chromatography
Cation Exchange Chromatography
types of resins
Specific Types
Silica
Controlled Pore Glass
Trade Names
Types of Ligands
Graft Chains
Mixed Mode Chromatography
Types ligands/supports
Hydroxyapatite
Different forms of
Operating Conditions
HCIC
Anion Mixed Mode
Cation mixed mode
Ligands and Linkers Generally
Filtration
Definitions Filtration
TFF
HPTFF
Particular Components
Virus Filtration
Filtration types
Depth Filters
Operating Conditions
Non-Antibody Purification
Enzyme purification
Interferon Purification
Milk Proteins
Virus Separation
Water Purification and Treatment
Waste Water Treatment
Purification Techniques
Partitioning
EOPO
PEG
Electrophoresis
Antibodies
antibody structure and types
constant region
Isotypes
IgA
IgG
catalytic antibodies
antibody fragments
single domain
bi-specific antibodies
BispAb Formats
monoclonal antibodies
humanized antibodies
polyclonal antibodies
Antibodies in Nature
Binding affinity
Antibody diversity
DNA rearrangement
Engineered antibodies
modified for binding affinity
pH dependent antibodies
Fc receptor modification
Altered Effector Function
Cell expression of Antibodies
Recombinant gene expression
eukaryotic systems
prokaryotic systems
Production Techniques
monoclonal antibody production
somatic cell hybridization
Transformation/Transfection B cells
phage display
Source of Antibodies
polyclonal antibody production
bi-specific antibody production & isolation
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therapeutic applications
specific disorders
complement components
Cytokines & Chemokines
diseases and disorders
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Telomere shortening
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Ear diseases
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Common agents of bioterrorism
Ishemia reperfusion injury
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Bacteria
Bacteria structure
Morphology
Gram +/- comparison
Medically important bacteria
Classification & detection
Enterobacteria
Motility assays
Selective media
Differential Media and Biochemical Tests
Bacterial metabolism
Disease mechanisms
Bacterial toxins
intestinal microbiota
MRSA & other dangerous bacteria
Medically important gram – bacteria
Legionella
legionella streaking
Legionella CFUs
host response to Legionella
Syphillis
Gram positive bacteria
Bacillus anthracis
Clostridium
Staphylococcus aureus
Streptococcus pyogenes
Rheumatic & scarlet fever
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Treatment for bacterial diseases
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Hemolytic anemia
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Treatment for cervical cancer
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Cancer genomics & proteomics
Chimeric antigen receptors (CARS)
Types of cancer
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Cancer disease mechanisms
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How viruses cause disease
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HIV structure
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Diagnosis of Eye Diseases
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Streptococcus pneumonia
COPD
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Lupus
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Immune Response
Adaptive Immunity
lymphoid organs
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Major histocompatibility complex (MHC)
MHC I molecules
MHC II molecules
Innate immunity
Defensins
Complement System
Functions of the complement system
Role of complement system in Disease
Complement receptors
Complement Pathways
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Lectin pathway
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C3 & C5
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Applications of Complement Regulators
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GVHD
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DNA
DNA repair
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Genetics
Recombination
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SNPs
DNA replication
Protein structure
Amino acid table
Prions
Transcription
Regulation of transcription
RNA capping & splicing
Alterations in chromatin structure
Methylation & demethylation
Histone acetylation
Transcription factors
NF-kB
Inhibitors of NF-kB
Activation of NF-kB
Translation
Immune cells
white blood cells/leukocytes
monocytes
dendritic cells
cytokines & chemokines produced
DC subsets in humans
DC subsets in mice
inhibition of DC maturation
maturation stimuli
T cell priming by DCs
DC development
factors affecting DC development
Role of DCs in tolerance
plasmacytoid DCs
DC surface markers
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Dendritic cells as regulators of T regs
lymphocytes
T cells
CD4+ T helper cells
TC (cytotoxic) Cells (CD8 cells)
Memory T cells
regulatory T cells
generation of T regs
Natural Tregs (D4+CD25+)
T suppressor (Ts)
Tr1 cells
T helper type 3 cells (Th3)
T cell receptor
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T cell functions
T cell repertoire
T cell activation assays
T cell development
B cells
B cell activation
B cell isolation
B cell development
B cell maturation
B cell tolerance
granulocytes
Neutrophils
Natural Killer Cells
Macrophages
Biochemistry
Aging
AIRE
Acids, Bases & Buffers
Buffers
Buffer conditions
Bonds
Hydrocarbons
Isomers
Hormones
Types of hormones
Regulation of digestion and obesity
Glycosylation
Structures & Types
Characterization & Detection
Functions of glycosylation
Metabolism
Bacterial metabolism
Photosynthesis
Lipoproteins
Oxidation & Reduction
Polymers
Cationic polymers
Flavoproteins
Evolution
Enzymes
Amylases
Endonucleases
CRISP
CRISP/Cas9
Glycosyltransferases
Glycosidase
Neuraminidases
Oxidoreductases
Polymerases
Topoisomerases
Proteases
Protease Inhibitors
Serine Protease Inhibitors
Lipolytic Enzymes
Bioinformatics
Portal entry sites
DNA/RNA bioinformatics
analysis of the genome
functional assessment of non-coding DNA
Glycomics
Metabolomics
Protein bioinformatics
structural characterization of proteins
De novo-designed proteins
protein function
phosphoproteome
Cancer genomics & proteomics
Single cell analysis & spacial omics
Cell Biology
Prokaryotes
Types of cells
Non-immune cells
Stem cells
Cell adhesion
Cell junctions
Integrins
Cell Development
Apoptosis
Activation of apoptosis
Caspases
Cell cycle
Cell cycle control
Cell membranes
Transmembrane transport proteins
Ion channels
Ligand gated
Voltage gated channels
Chromosomes
Cystoskeleton
Organelles
Mitochondria
Endoplasmic reticulum
Endosomes
Fertilization
Embryonic Development
Embryogenesis
Gene Therapy
CRISP Gene Editing
Rare Diseases
Vaccines & Immunization
Active Immunization
Immunogenicity
Ways to increase immunogenicity
Epitope based vaccines
Nucleic acid vaccination
RNA Vaccines
Dendritic Cell Vaccines
Drug Delivery
Hydrogels
Reversible gels
Gelatin
Non-Viral Vectors
Viral Vectors
Pharmacology
drug manufacture
Antibodies as drug carriers
Conjugation & Linkers
Cleavable Linkers/sites
Brain Drug Delivery
Gene delivery
signal transduction
Glycoproteins
Antibody glycoproteins
Lectins
ABO System
Glycoforms
Glycan Binding proteins
Siglets
DAMPS
PAMPS
Chimeric antigen receptors (CARS)
Fc receptors
B cell receptor (BCR) signalling
Viral sensing proteins
T cell receptor
G Protein coupled Receptors
G proteins
Cannabinoid receptors
Cannabis
Components of Cannabis
THC
Culturing techniques
Complement receptors
Enzyme Linked Receptors
Receptor tyrosine kinases
Receptor regulation
TLRs
Regulation of TLR signalling
Signaling through TLRs
TLR9
TLR4
TLR2
Class I Cytokine Receptor Family
Leptin Receptor
Ion channels
Voltage gated channels
Diagnostic techniques
Diagnostic Labs & equipment
Agglutination assays
Imunoagglutination
Hemagglutination
DNA diagnostic claims –DNA alles
Ariosa Diagnostics — Petition for rehearing en banc denied –Set up for the Supreme Court?
Ariosa Diagnostics – Diagnostic Method Claims
May 2016 Life Science Examples: Diagnostic claims: Example 29
Treatment Steps in Diagnostic claims
Cleveland Clinic — Diagnostic claims
Diagnostic assays with CRISPR
bioterrorism
Common agents of bioterrorism
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Science through the eyes of patents
Science through the eyes of patents
March 1, 2017
Science through the eyes of patents …
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