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Antibody Purification
Antibody purificaiton schemes
Affinity chromatography of antibodies
specific affinity ligands
protein A/C
conditions
secondary steps
virus inactivation
Fc receptors as affinity ligands
by HIC
Immunoaffinity of antibodies
Peptides and Mimotopes
By Hydrophobic Resins & Activated Carbon
By filtration
Membrane absorbers
carbon filters
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By ion exchange
AEX
CEX
by mixed mode chromatography
by hydroxyapatite
by HCIC
by partitioning
by precipitation
affinity precipitation
polyelectrolyte
Anionic
Cationic
Polymer precipitation
smart polymers
particular types of antibodies
antibody fusion proteins
Avion antibodies
IgY isolation
Precipitation agents
ammonium sulfate
IgA
domain antibodies
bi-specific antibodies
Particular contaminants purification
aggregates
charge variants
glycated variants
Extra Light Chains
Oxidized and Reduced Variants
Particular Sources for purification
Inclusion bodies
bioterrorism
Common agents of bioterrorism
Analytics & Characterization
next generation sequencing
Common Techniques
Light scattering
Mass spectrometry
Capillary electrophoresis
Plasma Proteins
fractionation procedures
Definitions
agents used
silicon dioxide
Fatty acids
Specific Plasma Proteins
albumins
IVIG
ethanol fractionation
ethanol precipitation Steps
chromatography
anion exchange of plasma proteins
cation exchange of plasma proteins
commercial preparations
diagrams
virus inactivation
aggregate/enzyme removal
IgM
IgA
Factor H
Protease Inhibitors
Inter-alpha-Inhibitor Proteins
A1PI
Chromatography
Definitions
Buffers
Column Arrangement or Use
Monoliths
expanded bed chromatography
In-line or Tandem Chromatography
Blood Purification
Batch Chromatography
continuous chromatography
Moving Bed
conditions and modes
displacement chromatography
weak partitioning mode
Overload Chromatography
purification schemes
Affinity Chromatography
IMAC
Lectin Affinity Chromatography
Immunoaffinity
Protein A
Structure
Analogues of Protein A
Temperature senstive mutants
Multimers of SpA domains
Parameters
elution
Resins
methods of coupling ligands to resin
Linkers
Regeneration
Protein L
Protein G
HIC
Conditions for HIC
RPLC
Small Molecules
Ion-Exchange Chromatography
Anion Exchange Chromatography
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types of resins
Specific Types
Silica
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Trade Names
Types of Ligands
Graft Chains
Mixed Mode Chromatography
Types ligands/supports
Hydroxyapatite
Different forms of
Operating Conditions
HCIC
Anion Mixed Mode
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Ligands and Linkers Generally
Filtration
Definitions Filtration
TFF
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Particular Components
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Operating Conditions
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Purification Techniques
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PEG
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antibody structure and types
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Isotypes
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antibody fragments
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complement components
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intestinal microbiota
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Biochemistry
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Acids, Bases & Buffers
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Regulation of digestion and obesity
Glycosylation
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Characterization & Detection
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Epitope based vaccines
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Drug Delivery
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Pharmacology
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Antibodies as drug carriers
Conjugation & Linkers
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Glycoproteins
Antibody glycoproteins
Lectins
ABO System
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Glycan Binding proteins
Siglets
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Chimeric antigen receptors (CARS)
Fc receptors
B cell receptor (BCR) signalling
Viral sensing proteins
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G Protein coupled Receptors
G proteins
Cannabinoid receptors
Cannabis
Components of Cannabis
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Complement receptors
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Receptor regulation
TLRs
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Signaling through TLRs
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Class I Cytokine Receptor Family
Leptin Receptor
Ion channels
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Diagnostic techniques
Diagnostic Labs & equipment
Agglutination assays
Imunoagglutination
Hemagglutination
DNA diagnostic claims –DNA alles
Ariosa Diagnostics — Petition for rehearing en banc denied –Set up for the Supreme Court?
Ariosa Diagnostics – Diagnostic Method Claims
May 2016 Life Science Examples: Diagnostic claims: Example 29
Treatment Steps in Diagnostic claims
Cleveland Clinic — Diagnostic claims
Diagnostic assays with CRISPR
bioterrorism
Common agents of bioterrorism
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February 9, 2017
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