Buffers
A buffer is a solution that resists changes in pH by the action of its acid-base conjugate components. Buffers act by releasing hydrogen ions when a base is added and…
Inclusion bodies
As to prokaryotic systems for the production of antibodies see Ypatent Health Definitions: Chaotropic agent: refers to a compound that in a sutiable concentraiton is capable of chaing the spatila…
HCIC
See also "purification schemes" under Antibody Purification. See also "mixed mode chromatography" under Chromatography Classification: HCIC is a type of mixed mode chromatographic process in which the protein of interest in the…
Cleveland Clinic — Diagnostic claims
Clevland Clinic Foundation v. True Health Diagnostics (Fed. Cir 2017) applies the two-step Alice framework to several patents of Cleveland Clinic and finds each patent invalid under the framework. US…
Bi-specific antibodies
See also bi-specific antibody production Bispecific antibodies can bind two different antigens. IgG type antibodies have two binding sites with different variable regions. An IgG variable region is made up…
Inter-alpha inhibitor proteins
Inter-alpha inhibitor proteins (IαIp) are a fmialy of structurally related serine protease inhibitors found at relatively high concentraitons (400-800 mg/L) in human plasma. Unlike other inhiibtor molecules, this fmaily consists…
Affinity chromatography of antibodies
See also "Protein A" under "affinity chromatography" See also Conditions for Affinity chromatography As to purification of antibody conjugates (e.g., Fc-fusion proteins) see "antibody conjugates" Affinity separation relies on the specific recognition between…
operating conditions for filtration
Commercial Viral Filters: Viresolve® See also Purification of plasma proteins, particularly virus inactivation. Operating Conditions Addition of Amino Acids: Hongo (US 13/260419) discloses a method of removing viruses from monoclonal antiboides…
HPTFF
High Performance tangential flow filtration (HPTFF): is where the protein separations are carried out on the basis of both size and charge (US 2003/0229212). HPTFF is thus a two-dimensional purification method which…