Cell detection & isolation
For immuno-detection see immuno-assays In many applications it is desirable to enrich or alternatively deplete certain cell populations in a sample. Hematopoietic cells and immune cells have been separated on…
Diagnostic techniques
Companies: Cepheid --STD testing: Everylwell (test for HIV and Syphilis, Hepatitis C, HIV, Herpes Type 2, Chlamydia, and Trichomoniasis Vaginalis at the same time; need a blood sample) It goes without saying that one…
B cells
see also B cell maturation and B cell activation B cells recognize native, non-processed antigen and generate antibodies in response to them in contrast to T cells which recognize processed antigen. B cells are antigen-presenting cells. The…
Natural Killer Cells
Derivation/Development: There are NK/T cell progenitors in bone marrow. There is a common T/NK progenitor cell. If this progenitor migrates to thymus, majority will rearrangement and become T cells. However,…
Neutrophils
Neutrophils comprises in humans 50-70% of all circulating leukocytes, whereas in mice they represent 10-25 of circulating leukocytes. (Rosales "Neutrophils at the crossroads of innate and adaptive immunity". J. Leukoc…
granulocytes
Granulocytes are innate immune cells that contain specialized granules to provide the first line of immune defense. Upon arrival at the site of infection, they participate in the inflammatory reaction…
B cell tolerance
Many diseases are characterized by pathogenic autoantibodies such as hemolytic anemia (erythroctye surface antigen), graves' disease (thyroid stimulating hormone R). There are several ways to deal with autoreactive immature B…
B cell maturation
Thymus-dependent (TD) activation: B cell activation can occur one of two ways. The first way depends upon TH cells and is called Thymus-dependent (TD). The other way does not and is referred to…
B cell development
See also antibody diversity B-cell development begins as lymphoid stem cells differentiate into progenitor B cells (pro-B cells) which express a transmembrane tyrosine phasphatase called CD45R. Proliferation and differentiation of these pro-B…
B cell isolation
One of the critical factors underlysing the difficulty in generating human Mabs is the low frequency of antigen specific B cells in the peripheral blood. Typically, antigen specific B cells…