Capillary electrophoresis

Capillary electrophoresis with laser induced fluorescence (CE-LIF): is a method for the direct quantitation of gene expression.  Detection and Quantification of Proteins Generally: Gavin (US 2015/0093757) disclsoes capillary electrophoresis methods which…

Protein identification

Mass spectrometry has become the method of choice for the Complex mixtures are separated before their MS analysis.

RT-PCR

PCR: Principles of PCR BonesLab (degenerate PCR)    Boehringer (RT-PCR)    GeneHunter (Differential Display)    Competitive PCR Real Time Primer Sets RealTime Probe & Primer Database Ambion (Real Time PCR basics) TRI REAGENT STEP (1) centrifuge samples for 10 min at 10C at 1100 RPMI   …

Factors to consider in PCR

Primer-DNA annealing temperature. This is generally dependent on the primer lenght and order and percentage of the nucleotides present composing the primers.  Number of cycles (the number of times that the…

PCR applications

Competitive PCR: is a a type of quantitative PCR. Exogenous target is added to create a competition between templates for amplification. The exogenous target is designed to amplify a smaller fragment…

PCR

Diagrams of PCR techniques PCR is a way to amplify DNA sequences even when they are present at only very small quantities. It is a technique invented by Kary Mullis…

Protein-DNA interaction

Technical Approaches toward identifying Proteins with DNA binding activities An important class of protein-protein interactions is that of proteins interacting with specific nucleic acid sequences, such as in the promoter…

Gene analysis

The analysis of gene expression is a common experiment conducted routinely in the laboratory. Profiling gene expression provides vlauable insight into genes involved in normal cell/tissue homeostasis, organisms development and…

Sample ELISA

IL-12 p40/p70 ELISA 1. Dilute capture anti-IL-12p40/p70 purified (10 ug/ml) in 0.1 M NaHCO3 (pH 8.2) and add 50 ul to appropriate # of weels of Coster EIA medium binding…

Macrophage ELISA

protocol Elisa macrophages obtain Peritoneal macrophages from BALB/c Mice. 1) inject mice with 3 ml thioglycolate broth (encourages macrophages to come to cavity) 2) wait 4 days 3) inject PBS Split cells…