Diagnostic assays with CRISPR
Pathogen detection Kits: Karius Digital™ culture kit ™ culture kit (tests for over 1,250 pathogens including bacteria and viruses) Diagnostic Assay related to the USE of CRISPR effector systems: CRISPR-Cas12 based…
Diagnostic Labs & equipment
See also Bacteria detection and Isolation under "bacteria" Cell Lines & Microorganisms: ATCC (American Type Culture Collection) Laboratory testing: Request a Test Quest Diagnostics (can order many tests here). STD Testing (find…
density separation
All density separation techniques have the same basic limitation; they can not separate subpopulations of cells with overlapping density distributions such as human lymphocyte subsets. To address this, dense particles…
Lables & conjugation
A wide variety of labels and conjugation techniques are known and reported extensively in both the scientific and patent literature. Preferred detectable labels include, but are not limited to, a fluorescent…
detection assays
Imaging Assays Axworthy teaches methods that relate to delivery of diagnostic agents (abstract). In one embodiment, a chemical conjugate or fusion protein with streptavidin (first member of a binding pair)…
Cell detection & isolation
For immuno-detection see immuno-assays In many applications it is desirable to enrich or alternatively deplete certain cell populations in a sample. Hematopoietic cells and immune cells have been separated on…
Diagnostic techniques
Companies: Cepheid --STD testing: Everylwell (test for HIV and Syphilis, Hepatitis C, HIV, Herpes Type 2, Chlamydia, and Trichomoniasis Vaginalis at the same time; need a blood sample) WasteWater Scan (many pathogens can be…
B cells
see also B cell maturation and B cell activation B cells recognize native, non-processed antigen and generate antibodies in response to them in contrast to T cells which recognize processed antigen. B cells are antigen-presenting cells. The…
Natural Killer Cells
Derivation/Development: There are NK/T cell progenitors in bone marrow. There is a common T/NK progenitor cell. If this progenitor migrates to thymus, majority will rearrangement and become T cells. However,…
Neutrophils
Introduction: Neutrophils comprises in humans 50-70% of all circulating leukocytes, whereas in mice they represent 10-25 of circulating leukocytes. (Rosales "Neutrophils at the crossroads of innate and adaptive immunity". J.…