B cell activation
See also antibody production methods B cell activation can occur one of two ways. The first way depends upon TH cells and is called Thymus-dependent (TD). The other way does not and is…
T cell activation assays
Proliferation Lymphoproliferative assay (LPA): largely measures the in vitro proliferative capacity of CD4T cells. Typically PBMC are placed in culture with recombinant protein antigens for 5-7 days and then pulsed with…
T cell repertoire
The TCR[beta] chain consists of variable (V), joining (J), diversity (D) and constant (C) regions encoded by gene segments spanning 685 kilobases of human chromosome seven. Diversity and antigen specificity…
T cell functions
TH cells orchestrate an acquired immune response by promoting intracellular killing by macrophages, antibody production by B lymphocytes, and clonal expansion of cytotoxic T lymphocytes. The TCRs of TH cells…
Naive T cells
Naive T cells: CD4 and CD8 T cells leave the thymus and enter circulation as resting cells in the G0 stage of the cell cycle. These naive cells express high levels…
T cell receptor
The TCR[beta] chain consists of variable (V), joining (J), diversity (D) and constant (C) regions encoded by gene segments spanning 685 kilobases of human chromosome seven. Diversity and antigen specificity…
T helper type 3 cells (Th3)
How they are generated Th3 are obtained after oral administration of antigen in vivo. In s, oral treatment with myelin basic protein (MBP) induces a significant increase in the frequency of…
Tr1 cells
Tr1 Cells How they are generated Tr1 cells are obtained by repetitive stimulation in vitro with IL10. IL-10 is required for the differentation of Tr1 cells, but is not sufficient when…
T suppressor (Ts)
Generation Like CD4+ TR cells, CD8+CD25- Ts cells can be generated in vitro aftermultiple rounds of stimulation of human PBMCs with either allogeneic or xenogeneic donor APCs. Similary, CD8+CD25= Ts…