density separation
All density separation techniques have the same basic limitation; they can not separate subpopulations of cells with overlapping density distributions such as human lymphocyte subsets. To address this, dense particles…
Lables & conjugation
A wide variety of labels and conjugation techniques are known and reported extensively in both the scientific and patent literature. Preferred detectable labels include, but are not limited to, a fluorescent…
detection assays
Imaging Assays Axworthy teaches methods that relate to delivery of diagnostic agents (abstract). In one embodiment, a chemical conjugate or fusion protein with streptavidin (first member of a binding pair)…
Cell detection & isolation
For immuno-detection see immuno-assays In many applications it is desirable to enrich or alternatively deplete certain cell populations in a sample. Hematopoietic cells and immune cells have been separated on…
Diagnostic techniques
Companies: Cepheid --STD testing: Everylwell (test for HIV and Syphilis, Hepatitis C, HIV, Herpes Type 2, Chlamydia, and Trichomoniasis Vaginalis at the same time; need a blood sample) WasteWater Scan (many pathogens can be…
B cells
see also B cell maturation and B cell activation B cells recognize native, non-processed antigen and generate antibodies in response to them in contrast to T cells which recognize processed antigen. B cells are antigen-presenting cells. The…
Natural Killer Cells
Derivation/Development: There are NK/T cell progenitors in bone marrow. There is a common T/NK progenitor cell. If this progenitor migrates to thymus, majority will rearrangement and become T cells. However,…
Neutrophils
Introduction: Neutrophils comprises in humans 50-70% of all circulating leukocytes, whereas in mice they represent 10-25 of circulating leukocytes. (Rosales "Neutrophils at the crossroads of innate and adaptive immunity". J.…
granulocytes
Granulocytes are innate immune cells that contain specialized granules to provide the first line of immune defense. Upon arrival at the site of infection, they participate in the inflammatory reaction…
B cell tolerance
Many diseases are characterized by pathogenic autoantibodies such as hemolytic anemia (erythroctye surface antigen), graves' disease (thyroid stimulating hormone R). There are several ways to deal with autoreactive immature B…