Cell expression of Antibodies

Introduction -Definitions: hybridoma fusion and cloning supplement (HFCS): is a low serum containing media supplement for the repalcemetn of fetal calf serum (FCS) in cultures of hybridoma cells. HFCS is specifically…
antibodystructure

Antibody Structure and Types

Figure from Nilsson (US6,740,734B1) Introduction: It is well documented that the structure of an immunogloublin forms a Y-shaped glycoprotein that is composed of two identical heavy and two identical light…

Protein G

Commercial Matrixs of Protien G Protein-G Sepharose 4 Fast Flow: is manufactured by GE Healthcare (Hober, J. Chromatogr B, 2007, 848, pp. 40-47 and contains SpG immobilized as a ligand.  Introduction…

Polymer precipitation

See also Plasma Protein Precipitation by Various Agents  Nonionic, water-soluble polymers induce protein precipitaiton by excluding water from the solvation structure of a protein. PEG is the most widely studied…

Depth Filtration

companies: 3M filtration See also depth filters generally Despite low shear centrifuge designs, the centrate effluent may still contain appreciable amounts of smaller sized contaminants (e.g., aggregates and colloids). Depth…

Fatty acids (e.g., caprylic acid)

See also Virus inactivation (caprylic acid can be used as an agent to do this) See also adjustment of pH with various agents under Agents used for fractionation See also AEX for…

Depth Filters

See also depth filtration for antibody purification Companies:  3 M Purification Depth filters refers to the use of a porous medium that is capable of retaining particulates throughout its matrix…

In-Line or Tandem

See also continous or SMB chromatography  In General:  Brown (WO2010/019148) discloses membrane chromatography run on either a standard chromatography system or a custom system like an AKTA exploer (GE Healthcare)…

Buffers

A buffer is a solution that resists changes in pH by the action of its acid-base conjugate components. Buffers act by releasing hydrogen ions when a base is added and…