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Distinguish Overload chromatography (see outline).  See also purification of antibodies using CEX displacement mode chromatography and overload chromatography  See also purification of antibodies using AEX displacement mode chromatography and overload chromatography Displacement chromatogrphy employs…

Batch Chromatography Traditionally, capture chromatography is run in single column, discontinous mode which is characterized in that the column is consecutively (i) equilibrated, (ii) loaded with feed containing the target…

See also antibody purification Definitions Ion-exchange chromatography: retention is based on the attraction between solute ions and charged sites bound to the stationary phase.  High-perfromance liquid chromatography (HPLC): is a…

Fluidized bed cchromatography involves the use of adsorbetn particles dispersed in a liquid medium. (Gagnon, J. Chromatogr. A 1221 (2012) 57-70). Expanded bed chromatogrpahy (EBA): EBA is a type of stabilized…

Monolithic stationary pahses consist of a porous polymer block. To create the polymer, monomer and cross linker molecules as well as molecules beraing the interactive groups (e.g., an ionomer in…

Continuous (simulated moving bed) chromatography:  See outline    As to monoliths see outline Companies:  Hosptial Supply Corporation In general, chromatographic methods chromatography columns are employed which are essentially comprising a column…

Amphiphilic: contain both hydrophobic groups ("tails") and hydrophilic groups ("heads"). Surfactants (detergents) are typically organic compounds that are amphiphilic which render surfactants soluble in both organic solvents and water.  Aqueous solution: refers to a…

See also Helpful Books in Resources:   The term chromatography refers to a process by which a solute of interest (e.g., protein of interest) in a mixture is separated from other solutes by…

MAbs undergo several posttranslational modifications such as disulfide bond formation, oxidation, deamidation, truncation and cyclization of N terminal glutamine residues to pyroglutamate. The heterogeneity observed in mAbs is often moniteored…