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The large-scale, economic purification of proteins is an increasinly important problem for the biopharmaceutical industry. Manufactuers of protein based pharmaceutical products must comply with strict regulatory standards, including extremely strinent…

Introduction: In this mode of chromatography, the product of interest is loaded beyond the dynamic binding capacity of the chromatography material for the product, thus referred to as overload.  In…

Weak partitioning mode of operation enhances the F/T mode by identifying solution conditions wehre there is weak binding of MAb to the resin (2 to 20 g/L). Under these conditions…

Distinguish Overload chromatography (see outline).  See also purification of antibodies using CEX displacement mode chromatography and overload chromatography  See also purification of antibodies using AEX displacement mode chromatography and overload chromatography Displacement chromatogrphy employs…

Batch Chromatography Traditionally, capture chromatography is run in single column, discontinous mode which is characterized in that the column is consecutively (i) equilibrated, (ii) loaded with feed containing the target…

See also antibody purification Definitions Ion-exchange chromatography: retention is based on the attraction between solute ions and charged sites bound to the stationary phase.  High-perfromance liquid chromatography (HPLC): is a…

Fluidized bed cchromatography involves the use of adsorbetn particles dispersed in a liquid medium. (Gagnon, J. Chromatogr. A 1221 (2012) 57-70). Expanded bed chromatogrpahy (EBA): EBA is a type of stabilized…

Monolithic stationary pahses consist of a porous polymer block. To create the polymer, monomer and cross linker molecules as well as molecules beraing the interactive groups (e.g., an ionomer in…

Continuous (simulated moving bed) chromatography:  See outline    As to monoliths see outline Companies:  Hosptial Supply Corporation In general, chromatographic methods chromatography columns are employed which are essentially comprising a column…

Amphiphilic: contain both hydrophobic groups ("tails") and hydrophilic groups ("heads"). Surfactants (detergents) are typically organic compounds that are amphiphilic which render surfactants soluble in both organic solvents and water.  Aqueous solution: refers to a…