columns used

Continuous (simulated moving bed) chromatography:  See outline    As to monoliths see outline Companies:  Hosptial Supply Corporation In general, chromatographic methods chromatography columns are employed which are essentially comprising a column…

Definitions

Amphiphilic: contain both hydrophobic groups ("tails") and hydrophilic groups ("heads"). Surfactants (detergents) are typically organic compounds that are amphiphilic which render surfactants soluble in both organic solvents and water.  Aqueous solution: refers to a…

Chromatography

See also Helpful Books in Resources:   The term chromatography refers to a process by which a solute of interest (e.g., protein of interest) in a mixture is separated from other solutes by…

glycated variants

See also Lectin Affinity Chromatography under Affinity Chromatography     See also antibody glycosylation in "biochemistry" under "glycosylation"   See also Characterization and Detection of Glycosylation The potential for significant changes in the biological activities…

charge variants

MAbs undergo several posttranslational modifications such as disulfide bond formation, oxidation, deamidation, truncation and cyclization of N terminal glutamine residues to pyroglutamate. The heterogeneity observed in mAbs is often moniteored…

aggregates

Protein aggregation is a common phenomenon that can be encountered during various stages of a commercial antibody manufacturing process. For example, aggregates can form during the fermentation, purification, final formulation…

Particular contaminants

As to charge variants see outline Therapeutic proteins can undergo a variety of degradation processess, including aggregation, deamidation, isomerization, oxidation, disulfide bond scrambling and truncation. These events can occur during fermentation,…

domain antibodies

Introduction Small genetically engineered immuoglobical constructs are being developed industry wide for a growing range of in vivo applications. Examples include Fab, F(ab')2, single-chain (sc) Fv, bis-scFV, diabodies, minibodies and…

IgA

See also purification of IgA from plasma Immunoglobulin A is the dominant immunoglobulin in human secretions including breast milk. Due to the low levels of IgA normally present in cows' milk,…

ammonium sulfate

Commercial Kits:  Pierce Chicken IgY Purification Kit   Gallus Immunotech  Promega Eggstract® Ammonium sulfate or sodium sulfate precipitation: has widely been used as an initial purification step in the isolation of immunoglobulin isotypes from serum…