Precipitation agents
Chloroform --Water dilution Chloroform: Larsen (US 12/299670 and WO2007/079755) discloses an alternative protocol for IgY isolation from egg yolk. First separate egg white and yolk, add 100 mM sodium phosphate buffer…
IgY isolation
See also extraction of IgY by precipitation Initial Extraction All IgY purification methods require an initial delipidation step to extract insoluble lipids and lipoproteins. Among the accepted commercial and laboratory delipidation…
Avion antibodies
Companies working with Avian Antibodies: Amicus Biotech Differences and Advantages of Avian Antibodies It is known that birds (e.g., laying hens) transfer their immunity to the yolk of their eggs and…
antibody fusion proteins
Affinity chromatography (Protein A/G) Affinity - IEX: Herrmann (WO 2005/100394A2) discloses a process for purifying IL-15/Fc fusion protein by applying the composition to an affinity chromatography column and applying the…
Particular Types
Isotypes as Particular Types of Antibodies Purified and Techniques Used: Antibody Fragments: (for Single Domain Binding Polypeptides and Nanobodies see "domain antibodies" in outline) --Advantages of Using Antibody Fragments: Small…
Particular Sources
For purification of other proteins from milk (which may involve same or similar steps) see Non-Antibody purification For purificaiton of antibodies and other proteins from Inclusion bodies see outline Separation of…
smart polymers
Conditions, such as solution pH, ionic strenght, solvent compoisition, termpature, light and electric field. These polymers are referred to as stimuli response, enivornmentally sensitive, "intelligent" or "smart" polymers. a large…
Cationic
Co-precipitation of non-IgG contaminants with positvely chartged polymers parallels the selectivity of AEX. These reagents selectively co-precipitate acidic host cell protins, DNA and varous cell culture additivies. (Pete Gagnon, J.…
Anionic
Anionic polyelectrolytes (polyanion polyelectrolytes) include copolymers of acrylic acid, methacrylic acid and methyl methacrylate (US 8362217), polyacrylic acid (PAA), polystyrenesulfonic acid (PSS, poly(4-vinylbenzenesulfonate metal soat)), polymethacrylate (PMA), polyacrylamidomethylpropanesulfonate (PAMPS), carboxymethylcellulose (CMC),…
polyelectrolyte
See also "smart polymers" and "partitioning" right hang panel. Polyelectrolytes are water-soluble polymers comprised of charged monomer units. The electrolyte (charged functionality) group undergoes protein dissociation (ionization) in aqueous solutions according…