aggregates
Protein aggregation is a common phenomenon that can be encountered during various stages of a commercial antibody manufacturing process. For example, aggregates can form during the fermentation, purification, final formulation…
Particular contaminants
As to charge variants see outline Therapeutic proteins can undergo a variety of degradation processess, including aggregation, deamidation, isomerization, oxidation, disulfide bond scrambling and truncation. These events can occur during fermentation,…
domain antibodies
Introduction Small genetically engineered immuoglobical constructs are being developed industry wide for a growing range of in vivo applications. Examples include Fab, F(ab')2, single-chain (sc) Fv, bis-scFV, diabodies, minibodies and…
IgA
See also purification of IgA from plasma Immunoglobulin A is the dominant immunoglobulin in human secretions including breast milk. Due to the low levels of IgA normally present in cows' milk,…
ammonium sulfate
Commercial Kits: Pierce Chicken IgY Purification Kit Gallus Immunotech Promega Eggstract® Ammonium sulfate or sodium sulfate precipitation: has widely been used as an initial purification step in the isolation of immunoglobulin isotypes from serum…
Precipitation agents
Chloroform --Water dilution Chloroform: Larsen (US 12/299670 and WO2007/079755) discloses an alternative protocol for IgY isolation from egg yolk. First separate egg white and yolk, add 100 mM sodium phosphate buffer…
IgY isolation
See also extraction of IgY by precipitation Initial Extraction All IgY purification methods require an initial delipidation step to extract insoluble lipids and lipoproteins. Among the accepted commercial and laboratory delipidation…
Avion antibodies
Companies working with Avian Antibodies: Amicus Biotech Differences and Advantages of Avian Antibodies It is known that birds (e.g., laying hens) transfer their immunity to the yolk of their eggs and…
antibody fusion proteins
Affinity chromatography (Protein A/G) Affinity - IEX: Herrmann (WO 2005/100394A2) discloses a process for purifying IL-15/Fc fusion protein by applying the composition to an affinity chromatography column and applying the…
Particular Types
Isotypes as Particular Types of Antibodies Purified and Techniques Used: Antibody Fragments: (for Single Domain Binding Polypeptides and Nanobodies see "domain antibodies" in outline) --Advantages of Using Antibody Fragments: Small…