by partitioning

See also partitioning in general under "partitioning" Both polyer/polymer and polymer/salt systems have been used for the purificaiton of antibodies. Using polymer/salt systems, IgG can be partially purified without using any…

by hydroxyapatite

Introduction Hydroxyapatite is commonly used for purification of antibodies Gagnon (US2008/0177048).  Hydroxyapatite is chemically similar to the mineral component of bones and hard tissues in mammals. it is seldom pure…

by mixed mode chromatography

Resins with multimodal ligands such as Capto MMC and Capto Adhere (GE Healthcare) can be used as polishing steps for antibody purificaiton. The multimodal functionality of the resin provides selectivity…

Antibody Purification by Cation Exchange

See also particular antibodies purified Introduction and What it is used for CEX has become a preferred method for IgG purificaiton. The relatively mild buffer solution conditions of CEX help…

AEX

See also cation exchange for purification of antibodies Anion exchange is nearly as universal as protein A in the purificaiton of monoclonal IgG. It is s employed frequently as the last…

By ion exchange

Ion exchange has several characteristics that make it particularly suitable for large scale purificaiton regimes, especially when compared to affinity based methods. IEX can be significantly cheapter than affinity matrices,…

carbon filters

Activated Carbon Filters Activated (active) carbon ("activated charcoal) is a form of carbon processed to have small, low-volume pores that inc4rease the surface area available for adsoption. it is produced…

Membrane absorbers

Membrane adsorbers are thin, synthetic, microporous or macroporous membranes that are derivatized with functional groups akin to those on the equivalent resins. The membranes are stacked 10-15 layers deep in…

By filtration

See also  Membrane Adsorbers See also filtration in conjunction with Protein A chromatography for the purification of antibodies See also  "Anion exchange" for membrane absorbers with anion exchange ligands. Companies:  disposable depth filter system: Pall, Cuno,…

Immunoaffinity of antibodies

See also Immunoaffinity generally under Affinity Chromatography Particular Types of Antibodies Used VHH antibodies: VHH which are naturally devoid of light chains can be used to generate immunosorbent materials for…