Antibody Purification by Cation Exchange
See also particular antibodies purified Introduction and What it is used for CEX has become a preferred method for IgG purificaiton. The relatively mild buffer solution conditions of CEX help…
AEX
See also cation exchange for purification of antibodies Anion exchange is nearly as universal as protein A in the purificaiton of monoclonal IgG. It is s employed frequently as the last…
By ion exchange
Ion exchange has several characteristics that make it particularly suitable for large scale purificaiton regimes, especially when compared to affinity based methods. IEX can be significantly cheapter than affinity matrices,…
carbon filters
Activated Carbon Filters Activated (active) carbon ("activated charcoal) is a form of carbon processed to have small, low-volume pores that inc4rease the surface area available for adsoption. it is produced…
Membrane absorbers
Membrane adsorbers are thin, synthetic, microporous or macroporous membranes that are derivatized with functional groups akin to those on the equivalent resins. The membranes are stacked 10-15 layers deep in…
By filtration
See also Membrane Adsorbers See also filtration in conjunction with Protein A chromatography for the purification of antibodies See also "Anion exchange" for membrane absorbers with anion exchange ligands. Companies: disposable depth filter system: Pall, Cuno,…
Immunoaffinity of antibodies
See also Immunoaffinity generally under Affinity Chromatography Particular Types of Antibodies Used VHH antibodies: VHH which are naturally devoid of light chains can be used to generate immunosorbent materials for…
by HIC
HIC is a well studied major polishing step in the purificaiton process of IgG based products and known for its capability to remove aggregated from of antibody. (Chen, J. Chromatography…
by HCIC
See also HIC and HCIC under "affinity chromatography" Hydrophobic charge induction chromatography (HCIC) is a type of mixed mode chromatography which as advatnages of mild elution conditionk high capacity and salt-toelrance.…
Fc receptors as affinity ligands
As to Fc receptors generally see Fc Receptors in Signal Transduction Fc receptor is a general term that refers to any one of several proteins that bind to the Fc region…