Editing genomes
See also CRISP gene editing under Enzymes and endonucleases Gene editing refers to the introduction of desired changes at specific genomic loci. The original strategy for gene editing was to use…
functional assessment of non-coding DNA
Once conserved non-coding sequences (CNSs) regions have been identified, the next step is to evaluate their function in biological experiments. There are various techniques to assess regulatory function. DNASE I…
analysis of the genome
Information used to predict genes includes signals in the sequence, content statistics and similarity to known genes. As noted by Attwood (Science, 290, 2000), there are many obstacles to accurate…
DNA/RNA bioinformatics
Cross-References: Protein Bioinformatics Genome Bioinformatics =========================================================================== Annotation: Seqvista Condons: Codon Table ESTs/Tandom Repeats: EST Extractor EST Sequence Database (NCBI) TGEM EST Assembly Machine Immunoglobulins: IMGT (links of CDR analysis and sequence alignment) Primers: Primer3 Primer3 (MIT) Primer generator for mutagenesis Tandom Repeat…
Bioinformatics
Bioinformatics is the application of information technology to the management and analysis of biological data with the aid of computers. Bioinformatics can be divided into 3 major divisions: 1) DNA/RNA,…
MRSA & other dangerous bacteria
See also bioterrorism agents Superbugs or bacterial strains which are resistant to antibiotics are becomming a major problem, particularly in hospitals. MRSA alone (below) causes more death each year than…
intestinal microbiota
The intestinal microbiota is confined most to the colon where some 1.5 kg of microbes or 1014 microorganisms reside. There are more genes in the human microbial than in the…
Bacterial toxins
Secretion of toxins is s a primary way bacteria cause disease. Toxins are bacterial components that directly harm tissue or trigger destructive biologic activities. One common delivery mechanism of such…
Disease mechanisms
See also Antibiotic Resistance Mechanisms In order to successfully colonize the gut and cause infection or disease, bacteria (or pathogenic bacteria) have evolved multiple virulence factors in interaction with the…
Bacterial metabolism
See also metabolism The process of bacterial metabolism begins with hydrolysis of large macromolecules in the external cellular environment by specific enzymes. Small molecules produced by this hydrolysis (i.e, fatty…