Bi-specific antibodies
See also bi-specific antibody production Bispecific antibodies can bind two different antigens. IgG type antibodies have two binding sites with different variable regions. An IgG variable region is made up…
Inter-alpha inhibitor proteins
Inter-alpha inhibitor proteins (IαIp) are a fmialy of structurally related serine protease inhibitors found at relatively high concentraitons (400-800 mg/L) in human plasma. Unlike other inhiibtor molecules, this fmaily consists…
Affinity chromatography of antibodies
See also "Protein A" under "affinity chromatography" See also Conditions for Affinity chromatography As to purification of antibody conjugates (e.g., Fc-fusion proteins) see "antibody conjugates" Affinity separation relies on the specific recognition between…
operating conditions for filtration
Commercial Viral Filters: Viresolve® See also Purification of plasma proteins, particularly virus inactivation. Operating Conditions Addition of Amino Acids: Hongo (US 13/260419) discloses a method of removing viruses from monoclonal antiboides…
HPTFF
High Performance tangential flow filtration (HPTFF): is where the protein separations are carried out on the basis of both size and charge (US 2003/0229212). HPTFF is thus a two-dimensional purification method which…
TFF
Tangential Flow Filtration (TFF or crossflow filtration) refers to a filtration process where the feed stream passes parallel to the membrane face as one portion passes through the membrane (permeate)…
modes of filtration
The following are commonly used modes of filtration. 1. Direct Flow Filtration (DFF): or "dead-end" filtration is where the feed stream is applied perpendicular to the membrane face, attempting to pass…
ultrafiltration
Ultrafiltration is a form of membrane filtration in which hydrostatic pressure forces a liquid against a semipermeable membrane. Suspended solids and solutes of high molcular weight are retained, while water and…
debth filters
Depth filters refers to the use of a porous medium that is capable of retaining particulates throughout its matrix rather than just on its surface. These filters are frequently employed…